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酵母双杂交筛选原癌基因proto-Dbl的上游调控分子

唐秋琳, 赵晓斐, 陈向征, 刘 明, 陈 济, 毕 锋

唐秋琳, 赵晓斐, 陈向征, 刘 明, 陈 济, 毕 锋. 酵母双杂交筛选原癌基因proto-Dbl的上游调控分子[J]. 肿瘤防治研究, 2010, 37(05): 499-502. DOI: 10.3971/j.issn.1000-8578.2010.05.003
引用本文: 唐秋琳, 赵晓斐, 陈向征, 刘 明, 陈 济, 毕 锋. 酵母双杂交筛选原癌基因proto-Dbl的上游调控分子[J]. 肿瘤防治研究, 2010, 37(05): 499-502. DOI: 10.3971/j.issn.1000-8578.2010.05.003
TANG Qiu-lin, ZHAO Xiao-fei, CHEN Xiang-zheng, LIU Ming, CHEN Ji, BI Feng. Screening of Upstream Regulators of Proto-Dbl Oncoprotein by Yeast Two-hybrid System[J]. Cancer Research on Prevention and Treatment, 2010, 37(05): 499-502. DOI: 10.3971/j.issn.1000-8578.2010.05.003
Citation: TANG Qiu-lin, ZHAO Xiao-fei, CHEN Xiang-zheng, LIU Ming, CHEN Ji, BI Feng. Screening of Upstream Regulators of Proto-Dbl Oncoprotein by Yeast Two-hybrid System[J]. Cancer Research on Prevention and Treatment, 2010, 37(05): 499-502. DOI: 10.3971/j.issn.1000-8578.2010.05.003

酵母双杂交筛选原癌基因proto-Dbl的上游调控分子

详细信息
    通讯作者:

    毕 锋

  • 中图分类号: R733.4

Screening of Upstream Regulators of Proto-Dbl Oncoprotein by Yeast Two-hybrid System

  • 摘要: 目的:探讨VL domain对Dbl癌基因活性的调控机制。方法:本文通过酵母双杂交的方法,以proto-Dbl的VL domain为诱饵蛋白对人胎脑文库进行筛选。结果:研究共获得7个能与VL domain相互作用的蛋白。结论:Proto-Dbl VL domain相互作用蛋白的发现将为揭示proto-Dbl的功能奠定基础。

     

    Abstract: Objective:The VL domain truncate mutation could directly cause the activation of proto-Dbl and the malignant transformation. Methods:In order to clarify the activation mechanism of proto-Dbl, yeast two-hybrid system was used to screen the proteins interacted with the VL domain in the human fetal brain cDNA library taking VL domain as the bait. Results:7 positive clones which interact with proto-Dbl-VL domain were successfully harvested. Conclusion:This discovery lays the foundation for uncovering of the regulatory mechanism of proto-Dbl.

     

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出版历程
  • 收稿日期:  2008-12-19
  • 修回日期:  2009-05-13
  • 刊出日期:  2010-06-24

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