Abstract: Objective To evaluate the Application of detecting Hodgkin's Lymphoma(HL) tumor cell and background cell with the combination of double-labeling with immunohistochemistry (IHC) and Later Capture microdissection (LCM). Methods This study includes 10 patients with HL diagnosed,tissue biopsy sample were fixed 10% formalin, paraffin-embedded, adopted different methods of antigen retrieval. The same sample were labeled with double labeling with IHC(CD20/CD45RO,CD30), then single tumor cell and background cell can get through LCM. Results In 10 cases of HL,tumor cells and background cells were detected with the combination of double labeling and LCM, they might show different color. The expression of tumor cell was CD30-postive with brown-yellow on cell membrane, and background cells were CD20-positive with red on cell membrane. Conclusion We can tell single tumor cells and background cell with double labeling and LCM, thus double labeling might play distinguish between different cells in HL.