Abstract: Objective To study the expression of PTEN, PI3K and Paxillin in esophageal squamous cell carcinoma（ESCC）and to investigate the relationship between the expression of PTEN,PI3K and Paxillin and carcinogenesis and progression of esophageal carcinoma and correlation among PTEN,PI3K and Paxillin expression each other . Methods PTEN,PI3K and Paxillin expression were detected in 24 normal esophageal mucosa, 94 ESCC with SP immunohistochemistry method. Results There were significant differences of PTEN,PI3K and Paxillin expressions between esophageal carcinoma and normal mucosa epithelium (all P<0.01). There were significant correlation between PTEN expression and the degrees of differentiation (P<0.05),invasive depth (P<0.05), clinical staging (P<0.05) and the metastasis of lymph node (P<0.01). There were significant correlation between PI3K expression and the degrees of differentiation,invasive depth, clinical staging and the metastasis of lymph node (all P<0.01).The positive expression rate of Paxillin had the relationship with invasive depth, clinical staging and metastasis of lymph node (all P<0.01). There was a negative relationship between PTEN and PI3K or Paxillin expression (P<0.01). A positive correlation was found between the expression of PI3K and Paxillin (P<0.05) . Conclusion PTEN,PI3K and Paxillin play important roles in carcinogenesis and progression of esophageal carcinoma，and can be used as valuable biomakers to evaluate biological characteristics in esophageal carcinoma. PTEN and PI3K may counteract with each other; PI3K and Paxillin may cooperate to promote malignant progress of esophageal carcinoma.