Abstract: Objective To explore the effect of RASSF1A on the chemosensitivity of human hepatocellular carcinoma (HCC) cell line QGY-7703. Methods The QGY-7703 cells expressing RASSF1A gene (wild type or mutant) stably were used in this study. The cells were treated with the antitumor drugs including Mitomycin, Adriamycin, Etoposide, 5-Fluorouracilur and Cisplatin. Then the rate of cell growth inhibition, changes of cell cycle and expression levels of p53, p21, bax and caspase-3 were measured. Results Expression of wild-type RASSF1A in the QGY-7703 cells could enhance the rate of cell growth inhibition, the percentage of apoptotic cells and caspase-3 activity compared with the cells that express mutant RASSF1A after those cells were treated with Mitomycin（P<0.05）. No significant differences of expression levels of the Bax, p53 and p21 protein were observed among those cells. Conclusion Wild-type RASSF1A expression could increase chemosensitivity of QGY-7703 cells to Mitomycin.