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云南元宝枫黄酮诱导个旧肺鳞癌细胞YTMLC凋亡的实验研究

Flavone of Yunnan Purpleblow Maple Is a Potent Apoptosis Inducer in YTMLC Cells

  • 摘要: 目的 研究云南元宝枫黄酮对个旧肺鳞癌细胞(YTMLC)凋亡诱导作用及相关基因表达的影响,为开发应用元宝枫黄酮提供实验依据。 方法 采用四甲基偶氮唑蓝(MTT)比色法检测元宝枫黄酮对YTMLC细胞的生长抑制作用,流式细胞术TUNEL法检测元宝枫黄酮对YTMLC细胞凋亡的影响,RT-PCR检测元宝枫黄酮对YTMLC细胞 p53、p21、bcl-2、bax和caspase-3基因表达的影响。 结果 元宝枫黄酮作用YTMLC细胞48 h后,IC50为(108.53±8.22)mg/L。流式细胞术显示元宝枫黄酮既可以诱导YTMLC细胞凋亡,也可以引起YTMLC细胞坏死。元宝枫黄酮对p53、p21、bax和caspase-3的基因表达均有明显上调作用;对bcl-2基因表达有下调作用。 结论 云南元宝枫黄酮可以抑制个旧肺鳞癌细胞(YTMLC)体外生长,引起肿瘤细胞坏死,诱导肿瘤细胞凋亡,其诱导肿瘤细胞凋亡的可能机制是通过上调p53、p21、bax和caspase-3基因的表达,下调bcl-2基因表达。

     

    Abstract: Objective To evaluate the apoptosis-inducing effect of flavone(which was extracted from Yunnan purpleblow maple) on YTMLC (Yunnan Gejiu Lung Squamous Carcinoma Cells) cell line and related altered gene expression change, this study would provide experimental basis for the clinical use of lung cancer theraphy. Methods MTT(Methy triazolyl tetrazolium) assay was used to evaluate the growth inhibiting effects of the flavone on YTMLC cells. Flow cytometry TUNEL assay was applied to detect the apoptosis rate of YTMLC cells after incubation with the flavone. Apoptosis related gene expression of YTMLC cells was tested by reverse transcriptive polymerase chain reaction(RT-PCR). Results The flavone exhibited a growth inhibitory effect on YTMLC cells, with 50% inhibition concentration(IC50) value (108.53±8.22) mg/L, after incubated for 48 h. Flow cytometry showed that the flavone of Yunnan purpleblow maple induced both apoptosis and necrosis. While upregulating the expression of p53、p21、Bax and Caspase-3, the flavone also downregulated the expression level of Bcl-2. Conclusion The flavone of Yunnan purpleblow maple can inhibit the growth of lung cancer cell (YTMLC) in vitro, and leads to apoptosis and necrosis of the tumor cells. The expression levels of apoptosis-related genes were altered, which implies its mechanism.

     

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