Abstract: Objective To investigate the relationship and action among theexpressions ofPTEN, NFκBandKi-67andgastric cancer. Methods The immunochemistry method was used to detect expressions ofPTEN,NFκB and Ki-67 protein. Results The positive rates of PTEN protein expressions in gastric cancer tissue were 24.2%（15/62）,obvious lower than those in par-cancer tissue,77.3%（17/22） (P<0.001). However, the positive rates of NFκB and Ki-67 protein expressions in gastric cancer tissue were61.3%（38/62）and 67.7%（42/62）,respectively, obvious lower than those in par-cancer tissue, 18.2%（4/22）and 13.6%（3/22）（P<0.01，P <0.001）. The positive rates of PTEN,NFκB and Ki-67 protein expressions were relation to clinical pathology stage,differentiation degree of tissue and survival periods of five years, but were independent ofsex and ages（P>0.05）; The patient that had lower clinical pathology stage and higher differentiation degree of tissue would had attained longer survival periods, the differences were significant(P<0.05). Moreover, in gastric cancer tissue, PTEN and NFκB manifested negative relationship（χ²=6.519，P<0.05）; PTEN and Ki -67 manifested negative relationship （χ²=4.022，P<0.05）,too. On the contrary, NFκB and Ki-67 manifested positive relationship（χ²=5.641，P<0.05）. Conclusion Missing actives of PTEN gene andNFκB gene activation maybe promoted a progress of origin, growth, soakage and metastasis of gastric cancer. Expressions of Ki-67 gene not only impersonally reflected multiplication activation of gastric cancer, but also estimated latency fatalness of tumour cell occurring metastasis，which could be used as a marker of clinic to appraise tumour's dangerdegree and prognosis.