Abstract: Objective To observe specific immune response and anti-tumor immunotherapy effect of the co -expression genetic vaccine MAGE/IL-18. MethodsCo-expression genetic vaccine was injected to mice,two booster injections were carried out at ten days intervals.Using pcMAGE-1,pcIL-18,pcDNA3 and PBS as comparison,IL-12，IFN-γ，CTL killing activity and T cell subgroup were investigated.Tumor growth and survival rates of vaccine immunized MAGE(+) mice were recorded. Results The mice which were immunized by pcIL-18-MAGE,compared with control groups,had the highest CTL killing activity,CD3⁺,CD4⁺,CD8⁺ T cells,IL-12 and IFN-γ all step up obviously.The co-expression vaccine can delay tumor growth time and prolong the live time for mice. Conclusion Co-expression genetic vaccine pcIL-18-MAGE can induce conspicuous and has specific anti-tumor immunotherapy effect on laboratory animals,and can significantly reduce the tumor growth.