Abstract: Objective To investigate the effects of ampelopsin(APS) on the growth of human gastric cancer cell SGC-7901 in nude mice. Methods SGC-7901 cells were inoculated into the nude mice to establish gastric cancer model.Then the BALB/C nude mice with xenograft tumor were randomized into 5 groups.The ampelopsin was administered at 3 dosages by irrigate the stomach daily for 10 d.The anti-tumor effects of ampelopsin was evaluated based on the tumor volume，relative tumor volume(RTV)，tumor weight，relative tumor proliferative rate （T/C）and tumor inhibition rate(IR). ResultsAt the 600mg/kg,300mg/kg,150mg/kg dose of APS,the relative tumor proliferation rate was 52.84％,71.12％ and 62.14%,respectively,and the growth inhibition of the transplanted tumor was 38.8％,27.5% and 27.3%,respectively. Conclusion APS in appears to be a potent anti-tumor agent that was firstly discovered in the transplanted human gastric cancer cell SGC-7901 in nude mice in vivo.