RNAi沉默survivin表达对A549细胞凋亡及紫杉醇敏感性的影响

董俊红; 王振明; 付新华; 王守训; 黄焕生

doi:10.3971/j.issn.1000-8578.2010.03.006

RNAi沉默survivin表达对A549细胞凋亡及紫杉醇敏感性的影响

Effects of Silence survivin by RNAi on Apoptosis of A549 Cells and on Sensitivity of A549 Cells to Paclitaxel

摘要

摘要: 目的利用RNAi （RNA interference,RNAi）沉默抗凋亡基因survivin，观察其对人肺腺癌细胞A549 凋亡以及紫杉醇药物敏感性的影响。方法构建重组质粒，将其导入A549细胞，检测转染前后survivin的表达情况，TUNEL法检测细胞凋亡情况，MTT法检测转染后A549细胞对紫杉醇的敏感性变化。结果成功构建重组质粒。转染重组质粒后，survivin表达明显降低；细胞凋亡率增加。转染前紫杉醇对A549细胞的IC50为转染后的11.9倍，P<0.05。结论构建的重组质粒能有效抑制survivin基因表达，诱导细胞凋亡，增强A549细胞对紫杉醇的敏感性。

Abstract: Objective To study the effects of silence survivin by RNAi on the apoptosis of A549 cells and on the sensitivity of A549 cells to paclitaxel. Methods The recombinant plasmid were constructed.A549 Cells were transfected by recombinant plasmid.The levels of survivinexpression were measured before and after transfection.Cell apoptosis was detected by TUNEL method.The sensitivity of A549 cells to paclitaxel was evaluated by MTT after transfection. ResultsThe recombinant plasmid was successfully constructed.The A549 cells transfected with recombinant plasmid showed lower expression of survivin.The apoptosis rate of A549 cell was increased after transfection.The IC50 of paclitaxel to A549 cells before transfection was 11.9 fold compared with those after transfection(P<0.05). Conclusion The recombinant plasmid could down-regulate the expression of survivin,induce apoptosis and enhance the sensitivity of A549 cells to paclitaxel.

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