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STAT3反义寡核苷酸对乳腺癌MCF-7细胞增殖和凋亡的影响

陈 佳, 徐 华, 张蕴莉

陈 佳, 徐 华, 张蕴莉. STAT3反义寡核苷酸对乳腺癌MCF-7细胞增殖和凋亡的影响[J]. 肿瘤防治研究, 2010, 37(03): 263-265. DOI: 10.3971/j.issn.1000-8578.2010.03.005
引用本文: 陈 佳, 徐 华, 张蕴莉. STAT3反义寡核苷酸对乳腺癌MCF-7细胞增殖和凋亡的影响[J]. 肿瘤防治研究, 2010, 37(03): 263-265. DOI: 10.3971/j.issn.1000-8578.2010.03.005
CHEN Jia, XU Hua, ZHANG Yun-li. Effects of STAT3 Antisense Oligonucleotide on Proliferation and Apoptosis of MCF-7 Breast Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2010, 37(03): 263-265. DOI: 10.3971/j.issn.1000-8578.2010.03.005
Citation: CHEN Jia, XU Hua, ZHANG Yun-li. Effects of STAT3 Antisense Oligonucleotide on Proliferation and Apoptosis of MCF-7 Breast Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2010, 37(03): 263-265. DOI: 10.3971/j.issn.1000-8578.2010.03.005

STAT3反义寡核苷酸对乳腺癌MCF-7细胞增殖和凋亡的影响

详细信息
    通讯作者:

    张蕴莉

  • 中图分类号: R737.9

Effects of STAT3 Antisense Oligonucleotide on Proliferation and Apoptosis of MCF-7 Breast Cancer Cells

More Information
    Corresponding author:

    ZHANG Yun-li

  • 摘要: 目的 探讨STAT3反义寡核苷酸的抗肿瘤机制,为乳腺癌的基因治疗提供新的实验依据。 方法 应用阳离子脂质体介导STAT3反义寡核苷酸转染乳腺癌MCF-7细胞,在倒置显微镜下观察细胞生长情况及形态变化;采用MTT比色法检测细胞增殖状态;流式细胞仪检测细胞凋亡率及细胞周期;Western blot检测STAT3总蛋白和p-STAT3蛋白的表达。 结果 STAT3反义核苷酸转染乳腺癌细胞后,能抑制MCF-7细胞的增殖,促进MCF-7细胞的凋亡,STAT3 蛋白的表达和磷酸化水平下降,出现G1期阻滞。 结论 STAT3反义核苷酸能抑制MCF-7细胞的增殖,促进MCF-7细胞的凋亡,这将为乳腺癌的治疗提供新的方向。

     

    Abstract: Objective To observe the influences of STAT3 antisense oligonucleotide on proliferation and apoptosis ofMCF-7 breast cancer cells.To study the effects of STAT3 antisense oligonucleotides on MCF-7 breast cancer cells in ovder to provide a new experiment evidence for breast cancer biological therapy. Methods The MCF-7 breast canner cell was transfected with STAT3 antisense oligonucleotide mediated by catholyte liposome.The cells growth and morphology were observed with a convert microscope.The proliferation of the cells was evaluated byMTT assay and the cell cycle was determined by flow cytometry.The expressions of total STAT3 protein and p-STAT3 protein were measured by western blot. Results The MCF-7 breast canner cell was transfected with STAT3 antisense oligonucleotide,which led to a sevies results including the inhibition of MCF-7 cell proliferation,the apoptosis of MCF-7 cell,the downregulation of STAT3 protein expression and phosphorylation,and the arrest at G1 phase. Conclusion STAT3 antisense oligonucleotides could inhibit MCF-7 cell proliferation,promote MCF-7 cell apoptosis,which may provide a new direction for the treatment of breast cancer.

     

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出版历程
  • 收稿日期:  2008-10-28
  • 修回日期:  2008-12-17
  • 刊出日期:  2010-04-24

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