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乏氧射线双调控的TK腺病毒载体联合放疗抑制乳腺癌裸鼠移植瘤的生长

魏 玲, 宋现让, 孙菊杰, 郑爱青, 王兴武, 谢 丽, 李 敏, 左文述

魏 玲, 宋现让, 孙菊杰, 郑爱青, 王兴武, 谢 丽, 李 敏, 左文述. 乏氧射线双调控的TK腺病毒载体联合放疗抑制乳腺癌裸鼠移植瘤的生长[J]. 肿瘤防治研究, 2010, 37(03): 259-262. DOI: 10.3971/j.issn.1000-8578.2010.03.004
引用本文: 魏 玲, 宋现让, 孙菊杰, 郑爱青, 王兴武, 谢 丽, 李 敏, 左文述. 乏氧射线双调控的TK腺病毒载体联合放疗抑制乳腺癌裸鼠移植瘤的生长[J]. 肿瘤防治研究, 2010, 37(03): 259-262. DOI: 10.3971/j.issn.1000-8578.2010.03.004
WEI Ling, SONG Xian-rang, SUN Ju-jie, ZHENG Ai-qing, WANG Xing-wu, XIE Li, LI Min, ZUO Wen-shu. Effects of TK Adenovirus Vector Regulated by Hypoxia and Radiation Combined with Radiotherapy on Growth of Transplanted Tumors of Breast Cancer in Nude Mice[J]. Cancer Research on Prevention and Treatment, 2010, 37(03): 259-262. DOI: 10.3971/j.issn.1000-8578.2010.03.004
Citation: WEI Ling, SONG Xian-rang, SUN Ju-jie, ZHENG Ai-qing, WANG Xing-wu, XIE Li, LI Min, ZUO Wen-shu. Effects of TK Adenovirus Vector Regulated by Hypoxia and Radiation Combined with Radiotherapy on Growth of Transplanted Tumors of Breast Cancer in Nude Mice[J]. Cancer Research on Prevention and Treatment, 2010, 37(03): 259-262. DOI: 10.3971/j.issn.1000-8578.2010.03.004

乏氧射线双调控的TK腺病毒载体联合放疗抑制乳腺癌裸鼠移植瘤的生长

详细信息
  • 中图分类号: R737.9;R730.55

Effects of TK Adenovirus Vector Regulated by Hypoxia and Radiation Combined with Radiotherapy on Growth of Transplanted Tumors of Breast Cancer in Nude Mice

  • 摘要: 目的 观察乏氧射线双调控的TK腺病毒载体-Ad.HRE.CArG.HSV-TK联合放疗对乳腺癌细胞Bcap37裸鼠移植瘤生长的影响。 方法 皮下接种细胞于BALB/C(nu/nu) 裸鼠建立移植瘤模型。当肿瘤直径达8~10mm时,将裸鼠分为对照组(control)、载体组(Ad)、照射组(RT)和载体合并照射组(Ad+RT)。治疗开始为第1 天,于第1、5天瘤内多点注射0.2ml表达载体,给药次日起腹腔注射GCV(40mg/kg),每日1 次,连续14天。第2、4、6天给予2Gy X线照射。每3天测量肿瘤长短径,计算肿瘤体积。治疗后30天处死裸鼠,剥离瘤块称重,计算抑瘤率。对肿瘤组织行HE染色和AnnexinV法检测凋亡。 结果 实验组平均肿瘤体积和瘤重均显著小于对照组(P<0.05)。HE染色可见肿瘤细胞变性坏死和凋亡细胞,AnnexinV检测显示实验组凋亡率明显高于对照组(P<0.05)。 结论 腺病毒载体联合放疗对Bcap37裸鼠移植瘤生长具显著抑制作用并可介导凋亡,为乳腺癌进一步的放射基因治疗研究奠定了基础。

     

    Abstract: Objective To study the effects of TK adenovirus vector-Ad.HRE.CArG.HSV-TK regulated by hypoxia and radiation combined with radiotherapy on the growth of transplanted tumors of breast cancer Bcap37 in nude mice. Methods The models of transplanted tumors in BALB/C(nu/nu) nude mice were established by subcutaneous inoculation cells.When tumor diameter increased to 8~10mm,the mice were allocated to be control group,vector group(Ad),radiation group(RT) and vector plus radiation group(Ad+RT).Day 1 was defined as treatment outset.On day 1 and day 5,0.2ml adenovirus vector was offered by tumor multi-point injection.The next day of injection,GCV(40mg/kg) was given using intraperitoneal injection,once on everyday for 14 days.X-ray irradiation of 2Gy was rendered on day 2,day 4 and day 6.The size of the tumor was measured every 3 days,and the volume of the tumor was calculated.After 30 days,the mice were killed,and the tumor was weighed and inhibition rate was calculated.HE staining and Annexin V detection were proceded to detect the apotosis of cancer cells in tumor tissues. Results The average volume and weight in experimental group were smaller than that in the control group(P<0.05).Necrosis and apoptosis were observed from the HE staining tissue.The rate of apoptosis in experimental group was significantly higher than that in the control group(P<0.05). ConclusionAdenovirus vector combined with radiotherapy had significant inhibition to the Bcap37 transplanted tumor in nude mice and induced cell.This provided the foundation for further gene radiation therapy of breast cancer.

     

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出版历程
  • 收稿日期:  2008-10-30
  • 修回日期:  2009-08-27
  • 刊出日期:  2010-04-24

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