Abstract: Objective To explore the change conditions of histone acetylation on proliferation of CNE2 cells inhibited by diallyl disulfide (DADS). Methods The growth inhibitions of CNE2 cell line were measured by MTT assay and cell counting.Expression of H3 and H4 was determined by western blot method. ResultsThe results from MTT assay and cell counting showed that DADS significantly inhibited the growth of CNE2 cells.Light microscope examination indicated that the heteromorphism and karyoplasmic ratio decreased.The results from Western blot showed that the acetylation level of histone H3 in CNE2 cell line increased by 20%,32%,42% and 30% compared to the control group,respectively,when treated with 90,140,240 and 400 μmol/L DADS for 48 h.The express of H3 exhibited a significant difference in the DADS does dependence.However,the acetylation level of histone H4 did not changed with a change of DADS does. Conclusion The diallyl disulfide (DADS) showed antiproliferation to the cultured human nasopharyngeal carcinoma CNE2 cell line,which is related to the over-expression of histone H3 resulted from DADS.