皮肤鳞状细胞癌FHIT基因的异常改变及意义

详细信息

Detection and Significance of Abnormality FHIT Gene in Cutis SCC

1.Department of Dermatology,The First Affiliated Hospital of Xinxiang Medical College,Weihui 453100,China；2.Department of Immunology,School of Basic Medical Science,Xinxiang Medical College；3.Department of Dermatology，The Second Hospital of Xi'an Jiaotong University

摘要: 目的检测脆性组氨酸三联体基因在皮肤SCC中外显子5和8的缺失和突变情况，分析该异常在皮肤SCC发生中的作用机制。方法 PCR-SSCP方法检测皮肤SCC患者皮损FHIT基因外显子5和8的缺失和突变状况。结果 10例CSCC组织中，E5有3例缺失，E8有8例缺失，其中有2例同时缺失E5和E8，对扩出的外显子进行SSCP分析没有检测到其突变。结论皮肤SCC中存在外显子5和8的缺失异常，没有发现其突变，这种异常与该肿瘤的发生可能有关，其具体的发生机制需做更进一步的探讨。
- 皮肤鳞状细胞癌 /
- FHIT /
- PCR-SSCP
Abstract: Objective To detect the deletion and mutation of exon 5 and 8 of fagile histidine triad gene （FHIT） in cutis SCC, and to analyze the role of the abnormality in the carcinogenesis of cutis cancer. Methods The deletion and mutations of exon 5 and 8 of FHIT gene were detected in 10 cutis CSCC tissues by PCR-SSCP methods. Results Deletion of exon 5 was observed in 3 out of 10 SCC tumor samples, and the deletion of exon 8 is 8 out of 10 CSCC. No mutation was found at exon 5 and 8 of FHIT gene. Conclusion Deletion of exon 5 and 8 was observed in cutis SCC and no mutation was found. The abnormality may play a role in the pathogenesis of cutis tumor SCC which deserve further research on the concrete pathogenesis.
- Cutis squamous cell carcinoma /
- FHIT /
- PCR-SSCP