Abstract: Objective This study aims to investigate the relationship between PI3K/AKt signaling pathway and the protection provided by LPA for cisplatin-induced apoptosis in ovarian carcinoma cells. Methods MTT assay was used to assess the proliferative activity of SKOV3 cells treated by DDP with or without LPA and LY294002. Hochest33258 was used to observe the apoptotic cells by fluorescence staining. FCM was used to detect the apoptosis of SKOV3 cells treated by DDP with LPA and LY294002. The specific DNA “ladder” pattern was used to confirm the apoptotic event. Western blotting was employed to detect the expression the levels of phosphorylated Akt protein. Results It revealed that LPA decreased the growth inhibition induced by DDP, but LPA+LY294002 increased the growth inhibition induced by DDP. In addition, it was also found that the apoptosis percentage in the cells treated with LPA+LY294002+DDP only exposed to LPA+DDP. Neither the apoptotic body nor the specific DNA “ladder” pattern for apoptotic cells was found in the cells exposed to LPA, but they were seen in the cells which were exposed to LPA+LY294002.Western blotting showed that LPA increased the expression level of expression of phosphorylated Akt protein, but LY294002 decreased. the level of expression of phosphated Akt protein. Conclusion LPA inhibit the apoptosis induced by DDP through the PI3K/AKt signaling pathway.