Abstract: Objective To investigate the enhanced anti-tumor effect of the dendritic cells by lycium bararum polysaccharides. Methods Monocytes obtained form human peripheral blood were induced into immature DCs and mature DCs in vitro. The whole antigen of hepatocarcinoma cells was extracted by freeze thawing method and used to sensitize immature DCs; Effective T cells were harvested by mixed lymphology reaction, and the specificity that effective T cells killed target hepatocarcinoma cells was examined by the MTT method. The effects of lycium bararum polysaccharides on DCs' maturity,T cell activation,proliferation and anti-tumor were studied. Results Lycium bararum can promote the maturity of DCs, DCs with whole antigen of hepatocarcinoma cells can activate specifically T cells into effective T cells which can kill hepato carcinoma cells specifically while not MG-63 cells. polysaccharides alone had no significant effect on the proliferation of T cells but they could promote the maturity of DCs and reinforce proliferation and anti-tumor effects of effective T cells. Conclusion Lycium bararum polysaccharides can promote the maturity of DCs and reinforce proliferation and anti-tumor effects of effective T cells.