Abstract:
Objective To investigate the expression and significance of tumor suppressor gene FEZ1 protein and mRNA in gastric carcinoma. Methods Immunohistochemical staining(SP method)and reverse transcription-polymerase chain reaction(RT-PCR)were used to detect FEZ1 protein and mRNA expressions in 36 cases of gastric carcinoma and 36 cases of normal gastric epithelia.To analyze the relationship between the expression of FEZ1 and clinical data. Results The positive expression rate of FEZ1 protein in gastric carcinoma(55.6%)was significantly lower than that in normal gastric epithelia (86.1%)(
P<0.05). The expression of FEZ1 protein was closely related with degrees of differentiation(
P<0.05). The relative contents of FEZ1 mRNA in gastric carcinoma (0.677±0.120) was significantly lower than that in normal gastric epithelia (0.746±0.119).The relative contents of FEZ1 mRNA in gastric carcinoma was also correlated with degrees of differentiation and lymph node metastasis(
P<0.05). Conclusion The expression of FEZ1 has close relationship with the carcinogenesis and development of gastric carcinoma.