多抗甲素对树突状细胞瘤苗体外抗肿瘤活性的影响
Effect of PA on Antitumor Activity of DC Vaccine in vitro
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摘要: 目的 研究多抗甲素（Polyactin A,PA）对树突状细胞（Dendritic cell,DC ）瘤苗体外抗肿瘤活性的影响。方法 用GM-CSF、IL-4、TNF-α体外诱导扩增脐血单个核细胞（CBMCs）中的DC，24h后将凋亡、坏死的HeLa、HepG2细胞分别加入CBMCs共孵育，使DC接受肿瘤细胞全抗原刺激，成为DC瘤苗，之后再加入PA刺激活化DC瘤苗，3天后以CBMCs为效应细胞（另设未加PA组为对照），以相应的肿瘤细胞为靶细胞，用MTT法测定不同效靶比下效应细胞对HeLa、HepG2细胞的杀伤率。结果加入PA后，CBMCs对HeLa、HepG2细胞的杀伤率显著提高，明显高于未加PA组。结论PA可增强DC瘤苗对相应肿瘤的杀伤率。Abstract: Objective To evaluate the effect of polyactin A（PA）on the antitumor activity of dendritic cell(DC）vaccine in vitro. Methods Human cord blood mononuclear cells(CBMCs）were isolated and cultured with GM-CSF+IL-4+TNF-α for 24 hours to induce differentiation to DC.These CBMCs were incubated with apoptotic and necrotic HeLa / HepG2 tumor cells to vaccinate DC.These CBMCs were divided into two groups one group was cultured in the presence of PA,the other group was without PA as control 3 days later we measured the anti-tumor cytotoxicity of these CBMCs to HeLa / HepG2 by MTT assay. ResultsIn the presence of PA,the kill rate on the HeLa / HepG2 cells of the CBMCs was significantly higher than that of control group. ConclusionPA improves the antitumor activity of DC vaccine.
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