Abstract: To investigate the overexpression of NET-1、Ki67 and CD34 and clinicopathological significance in gastric carcinoma. Methods The overexpression of NET-1、Ki67 and CD34 was detected in 86 cases of gastric carcinoma paraffin-embeded sections by immunohistochemical method. Immunohistochemical trimarkers double staining method was used to detect all positive cases with the three markers and show the adjacent relationship and accompanying conditions between NET-1、Ki67 and CD34 on the one slide.All cases were followed up over 60 months. Results The overexpression rates of NET-1, Ki67 and CD34 in gastric carcinoma were 56.98％、74.42％ and 62.79％,respectively. There was significant positive correlation between these three markers (P <0.05). The overexpression rate of these markers were remarkably positive correlated with tumor clinical stages（P<0.05）and negatively correlated with tumor survival rate（P<0.05）and metastasis （P<0.05）. The survival rate within 3 and 5 years were dramatically shorter in the overexpression groups of these three markers than that in low expression groups and the negative groups（P<0.05，P<0.01） . The overexpression of NET-1 and Ki67 were negatively correlated with carcinoma differentiation (P<0.01). The overexpression of NET-1 and CD34 was positively correlated with infiltration and lymph node status of tumor (P<0.05), respectively. Conclusion The overexpression of NET-1, Ki67 and CD34 proteins was related with the proliferation, angiogenesis and poor prognosis in gastric carcinoma.