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低分子量姬松茸多糖对小鼠S180肉瘤的 抑制作用及其机制

牛英才, 赵学梅, 张春, 周丽, 刘吉成

牛英才, 赵学梅, 张春, 周丽, 刘吉成. 低分子量姬松茸多糖对小鼠S180肉瘤的 抑制作用及其机制[J]. 肿瘤防治研究, 2009, 36(03): 180-182. DOI: 10.3971/j.issn.1000-8578.2009.03.003
引用本文: 牛英才, 赵学梅, 张春, 周丽, 刘吉成. 低分子量姬松茸多糖对小鼠S180肉瘤的 抑制作用及其机制[J]. 肿瘤防治研究, 2009, 36(03): 180-182. DOI: 10.3971/j.issn.1000-8578.2009.03.003
NIU Ying-cai, ZHAO Xue-mei, ZHANG Chun, ZHOU Li, LIU Ji-cheng. Antitumoral Effect of Low Molecular Weight Polysaccharide Isolated from Agaricus Blazei Murill on Sarcoma 180 in Mice and Its Mechanism[J]. Cancer Research on Prevention and Treatment, 2009, 36(03): 180-182. DOI: 10.3971/j.issn.1000-8578.2009.03.003
Citation: NIU Ying-cai, ZHAO Xue-mei, ZHANG Chun, ZHOU Li, LIU Ji-cheng. Antitumoral Effect of Low Molecular Weight Polysaccharide Isolated from Agaricus Blazei Murill on Sarcoma 180 in Mice and Its Mechanism[J]. Cancer Research on Prevention and Treatment, 2009, 36(03): 180-182. DOI: 10.3971/j.issn.1000-8578.2009.03.003

低分子量姬松茸多糖对小鼠S180肉瘤的 抑制作用及其机制

详细信息
  • 中图分类号: R282.71; R730.51; R963

Antitumoral Effect of Low Molecular Weight Polysaccharide Isolated from Agaricus Blazei Murill on Sarcoma 180 in Mice and Its Mechanism

  • 摘要: 目的观察LMPAB对小鼠S180肉瘤生长的抑制及诱导凋亡的作用。方法BALB/c小鼠右后侧腋窝皮下接种S180瘤株后,腹腔分别注射50、100和200mg/(kg•d) LMPAB,连续7d,给药结束后24h处死小鼠,称肿瘤重量。Annexin-Ⅴ-FITC/PI双染法(流式细胞仪)检测肿瘤组织细胞凋亡。结果与模型组(1.038±0.262)比较,猪苓多糖组(0.704±0.262)和LMPAB高剂量组(0.695±0.096)肿瘤重量明显减轻(P<0.01)。猪苓多糖组、姬松茸粗多糖组、LMPAB低剂量组、LMPAB中剂量组和LMPAB高剂量组对S180肉瘤的平均抑制率分别为32%、23%、9.5%、23%和32%。与模型对照组比较,猪苓多糖组、姬松茸粗多糖组、LMPAB低剂量组、LMPAB中剂量组和LMPAB高剂量组的肿瘤细胞凋亡率明显增加,差异具有统计学意义(P<0.01)。结论LMPAB对S180肉瘤生长具有抑制作用,其机制可能是通过促进肿瘤细胞凋亡而实现的。

     

    Abstract: To observe antitumor activity and inducing apoptosis of low molecular weight polysaccharide isolated from Agaricus blazei Murill (LMPAB) on sarcoma 180 in mice. Methods The BALB/c mice inoculated in armpit with S180 cell suspension were treated by intraperitoneal injection of LMPAB [50 100 and 200 mg/(kg&#8226;d)] for 7days, The mice were sacrificed after 24h and tumor tissues were weighed respectively. Tumor cell apoptosis was detected by Annexin-Ⅴ-FITC/PI (Flow cytometry). Results In LMPAB group [200 mg/(kg&#8226;d)] (0.695±0.096) and Polyporus polysaccharide group (0.704±0.262) tumor weight reduced compared with the model group(1.038±0.262, P<0.01), Inhibitory rate of sarcoma 180 in mice treated with 50, 100, 200 mg/(kg&#8226;d) LMPAB was 9.5%, 23% and 32%, respectively. The apoptosis rate of sarcoma 180 cells in mice treated with 50, 100, 200 mg/(kg&#8226;d) LMPAB was (39.21±3.85) %, (32.17±5.10)% and (18.71±2.52)%, respectively, higher than that in the model group mice [(10.98±1.55)%]. Conclusion LMPAB significantly inhibited the growth of sarcoma 180 subcutaneously implanted in mice, possibly through inducing apoptosis of tumor cells.

     

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出版历程
  • 收稿日期:  2008-03-13
  • 修回日期:  2008-06-05
  • 刊出日期:  2009-04-24

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