Alpha B-crystallin基因在结肠癌组织中的表达及其意义

杭晓声; 汪道远; 王卫理; 李莉华

doi:10.3971/j.issn.1000-8578.2009.02.016

Alpha B-crystallin基因在结肠癌组织中的表达及其意义

1.214062 江苏无锡，苏州大学附属第四医院肿瘤科；2.上海瑞安肿瘤诊所，上海交通大学医学院附属上海瑞金医院；3.苏州大学附属第四医院普外科，4.实验室

详细信息

中图分类号: R735.3
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出版历程
- 收稿日期: 2008-01-21
- 修回日期: 2008-06-03
- 刊出日期: 2009-03-24

Alpha B-crystallin Expression and Its Significance in Colon Carcinoma Tissues

1. Department of Medical Oncology，Wuxi Cancer Hospital, 4th Hospital Affiliated to Suzhou University, Wuxi, 214062,China;2. AmMed Cancer Center, Shanghai Ruijin Hospital Affiliated to Medical School of Shanghai Jiaotong University;3. Department of General Surgery,Wuxi Cancer Hospital, 4th Hospital Affiliated to Suzhou University, 4.Department of Laboratory

摘要

摘要: 目的探讨alpha B-crystallin 基因在结肠癌组织中的表达及其与结肠癌临床病理的相关性。方法采用RT-PCR方法检测64例结肠癌组织及其癌旁正常组织中alpha B-crystallin 基因的表达，计算其阳性率；并用Western blot方法检测结肠癌组织及其癌旁正常组织中alpha B-crystallin蛋白的表达变化。结果 alpha B-crystallin mRNA在结肠癌组织中表达的阳性率明显高于癌旁正常组织(P<0.01),在发生浆膜外转移者的表达阳性率明显高于浆膜内者(P<0.05)，在较大肿瘤者中的表达阳性率明显高于较小肿瘤者，在有淋巴结转移者中的表达阳性率明显高于无淋巴结转移者(P<0.05),但与患者的性别、年龄、肿瘤的分化程度无关(P>0.05)。alpha B-crystallin蛋白在结肠癌组织中表达明显高于癌旁正常组织(P<0.01)。结论 alpha B-crystallin基因的高表达可能与结肠癌的发生及侵袭转移有关。
- Alpha B-crystallin /
- 结肠癌 /
- RT-PCR /
- Western blot
Abstract: Objective To study the expression of alpha B-crystallin in colon carcinoma, and its relation in clinical pathology of colon carcinoma. Methods The expression of alpha B-crystallin mRNA was detected using RT-PCR in 64 colon carcinoma tissues and adjacent normal tissues. The expression level of alpha B-crystallin protein was detected by RT-PCR and Western blot. The detection rates of alpha B-crystallin mRNA and protein expression in cancer tissues and adjacent normal tissues were analyzed. Results Alpha B-crystallin mRNA expression rate in cancer tissues was significantly higher than adjacent normal tissues (P<0.01). The patients with colon cancer involving the serosa obtained obviously higher alpha B-crystallin expression rate than the one not involving the serosa, The group which has bigger tumor or with lymph node metastasis has higher expression of alpha B-crystallin than those group with smaller size of tumor or without lymph node metastasis (P<0.05), and its expression has no correlation with age, sex and tumor differentiation (P>0.05). The protein of alpha B-crystallin was detected in cancer tissues but not in adjacent normal tissues (P<0.01) by Western blotting. Conclusion The expression of alpha B-crystallin is positively correlated with tumorogenesis and progression of colon cancer. It may be used as a good marker for clinic therapeutic purpose and prognosis estimation of colon cancer.
- Alpha B-crystallin /
- Colon Carcinoma /
- RT-PCR /
- Western Blot