Abstract: Objective To investigate the expression of CXCR4/CXCL12 and its potential role in hepatic metastasis of gastric carcinoma. Methods Gastric cancer tissue, adjacent normal tissue and hepatic metastatic tissue samples were collected from 40 gastric carcinoma patients. Several CXCR4/CXCL12 pathway-related proteins (CXCR4,CXCL12,cyclin D1 and Bcl-xL)expression were detected by Western blot. Correlations between CXCR4/CXCL12 expression and clinicopathological parameters in gastric carcinoma were analyzed. CXCR4 and CXCL12 expression in cell level were determined using immunohistochemistry method. Results The expression of CXCR4 and CXCL12 protein significantly increased in gastric cancer compared to adjacent normal mucosa (P<0.05), it was also highly expressed in metastatic tissue versus primary tumor tissue (P<0.05) Our data showed that expression of CXCR4 and CXCL12 protein are correlated with TNM colorectal cancer staging(P<0.05). Conclusion CXCR4/CXCL12 overexpressed in both primary and metastatic tissue of gastric carcinoma. It is implied from our results that CXCR4/CXCL12 signaling pathway plays an important role in hepatic metastasis of gastric cancer.