半边旗提取物5F对非小细胞肺癌NCI-H460细胞生长的抑制作用

Inhibitory Effects of 5F from Pteris semiinnataL. on Proliferation of Non-small Cell Lung Cancer NCI-H460 Cells

摘要: 目的研究半边旗提取物5F对非小细胞肺癌NCI-H460细胞生长的抑制作用。方法 MTT法检测5F对NCI-H460细胞的生长抑制作用；用PI-Hoechst荧光双染法和TUNEL法检测细胞凋亡；流式细胞仪检测5F对DNA含量的影响。结果 5F抑制NCI-H460细胞的生长，其效果与5F的浓度和作用时间相关，24、48、72h的IC₅₀分别为：21.40、4.52、1.02μg/ml；荧光双染色法显示经5F作用后细胞出现变形，染色质浓缩，产生凋亡小体；细胞被阻滞在G₂/M期。结论 5F在体外能有效地抑制NCI-H460细胞的生长，其作用可能是通过诱导其凋亡产生的。

Abstract: Objective To investigate inhibitory effects of 5F from Pteris semiinnataL. on proliferation of Non-small cell lung cancer NCI-H460 cells. Methods NCI-H460 cell growth inhibition mediated by 5F was detected by MTT. PI-Hoechst double staining and TUNEL assay were used to observe cell apoptosis. Flow cytometer was used to determine the effect of 5F on the content of DNA in cells. Results 5F displayed growth inhibitory activity against NCI-H460 cells with IC₅₀ values of 21.40,4.52 and 1.02 μg/ml at the point of 24, 48 and 72 hours. 5F could induce apoptosis. NCI-H460 cells treated by 5F were arrestet in G₂/M. Conclusion In vitro 5F significantly inhibited the proliferation of NCI-H460 cells and the activity might be realized through inducing apoptosis.