多西紫杉醇联合丝裂霉素C 抑制宫颈癌细胞 的增殖与迁移作用
Effect of Docetaxel Combined with Mitomycin C on Proliferation and Migration of HeLa Cells
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摘要: 目的 探讨多西紫杉醇(Doc) 联合丝裂霉素C (MMC) 抑制宫颈癌细胞的増殖与迁移作用。方法 MTT实验测定HeLa 细胞増殖能力,损伤修复实验测定细胞迁移速度。计算Q 值反映多西紫杉醇和丝裂霉素C 联合用药效果。结果 多西紫杉醇、丝裂霉素C 都能够抑制HeLa 细胞的増殖,作用具有浓度依赖性(Doc :0. 5μg/ L vs 0. 1μg/ L, P < 0. 05 ;MMC:10 μg/ L vs 2. 5 μg/ L, P < 0. 05) ;Q 值> 1, 显示两者联合用药有相加作用;同时损伤修复实验显示联合用药组细胞迁移速度(0. 035 μm/ h) 显著低于空白对照组(0. 173μm/ h, P < 0. 05) 及单独用药组(Doc 0. 079 μm/ h, MMC 0. 075 μm/ h) 。结论 多西紫杉醇和丝裂霉素C 能抑制宫颈癌细胞増殖与迁移,具有一定量效关系;两者联合用药具有协同作用,这可能与两者药效学靶点不同有关。Abstract: Objective To investigate effect of Docetaxel (Doc) combined with Mitomycin C (MMC) on pro2 liferation and migration of HeLa cells. Methods Proliferation and migration ability of HeLa cells were tested by MTT and wound healing assay respectively. The combined effect of Doc and MMC was evalua2 ted by“Q value”. Results The inhibition effect of Doc or MMC on proliferation of HeLa cells was dose dependent (Doc :0. 5μg/ L vs 0. 1μg/ L, P < 0. 05 ;MMC:10μg/ L vs 2. 5μg/ L, P < 0. 05) ;“Q value”ex2 ceed 1. 0, which indicated the synergistic effect of Doc and MMC. The migration velocity of cells in (Doc + MMC) group (0. 035μm/ h) was lower than Cont rol (0. 173μm/ h, P < 0. 05) group, Doc (0. 079μm/h) and MMC (0. 075μm/ h) group. Conclusion Doc and MMC dose2dependently inhibit proliferation and migration of HeLa cells respectively, and there was synergistic effect of the combination therapy. That may be on account of the different target s of two drugs.
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