Abstract: Objective To investigate whether the survivin antisense oligonucleotides could decrease the expression of survivin in vitro and sensitize human bladder transitional cell carcinoma T24 cells to recombinant TGF alpha-PE40( TP40). Methods The experiment cells were divided into five group,such as control group,survivin missense oligonucleotides group(MS),survivin antisense oligonucleotides group(AS),TP40 group and combined group(TP40+ AS).RT-PCR and Western blot assay was performed to detect the expression of survivin in all groups cells.MTT assay was applied to detect the cell proliferation of all groups.Flow cytometry were performed to detect TP40-triggered apoptosis.The tumor formation experiment of T24 cells in vitro was used to evaluate all groups cells' independent ability of anchoring growth. Results The survivin antisense oligonucleotides could down-regulate the expression of survivin at a concentration of 300nM.We have demonstrated from the mRNA level and protein level that the combined group could down-regulate the expression of survivin compared with the non-combined group powerfully.In the third day,the survival rate of the combined group cells was only 12.2%,the apoptotic rate was 96.37%,and the ability of tumor formation in vitro was very poor. Conclusion survivin antisense olignucleotides could sensitize human bladder transitional cell carcinoma T24 cells to TP40 with a cooperative effect.