抵抗素13肽对人乳腺癌细胞系MCF-7增殖和凋亡的影响

陈仲; 潘兵; 路璐; 赵明辉; 李德冠; 吴红英; 韩佩珍

doi:10.3971/j.issn.1000-8578.1840

抵抗素13肽对人乳腺癌细胞系MCF-7增殖和凋亡的影响

中国医学科学院中国协和医科大学放射医学研究所天津市分子核医学重点实验室; 中国医学科学院; 中国协和医科大学放射医学研究所天津市分子核医学重点实验室

详细信息

中图分类号: R737. 31
计量
- 文章访问数: 2140
- HTML全文浏览量: 27
- PDF下载量: 702
出版历程
- 收稿日期: 2007-04-15
- 修回日期: 2007-06-10
- 刊出日期: 2008-03-04

Effect of Resistin-13-peptides on Proliferation and Apoptosis of MCF-7 Cells in vitro

Tianjin Key Laboratory of Molecular Nuclear Medicine; Institute of Radiation Medicine; Chinese Academy of Medical Sciences and Peking Union Medical College; Tianjin 300192; China

摘要

摘要: 目的研究人工合成抵抗素13肽对人乳腺癌细胞系MCF-7增殖和凋亡的影响。方法根据人抵抗素cDNA编码的氨基酸序列合成抵抗素分子22—34位的13个氨基酸的多肽（CSMEEAINERIQE）。采用MTT法检测细胞的增殖活性，使用流式细胞仪检测细胞凋亡。结果50、500、5000和10000ng／ml抵抗素13肽均能抑制MCF-7细胞生长，抑制率为46％到92％，与对照组相比差异有统计学意义（P〈0．01）。诱导凋亡，四个浓度诱导的细胞凋亡率分别为6．7％、6.9％、7.5％和9．8％，与对照组0．6％的凋亡率比较P〈0．01。结论抵抗素13肽可以抑制乳腺癌细胞系MCF-7的增殖，促进细胞凋亡。
- 抵抗素 /
- 乳腺癌 /
- 增殖 /
- 细胞凋亡
Abstract: Objective 　To investigate the potential effect s of resistin2132peptides on the growth and apopto2 sis in human breast carcinoma cells, MCF27. Methods 　Basing on the deduced amino2acide sequence f rom the cDNA, a peptide of human resistin molecule, consisting of 13 amino2acide (CSMEEAINERIQE) was synthesized. 32(4, 52Dimethyl222thiazolyl)2 2, 52diphenyl22 H2tet razolium bromide (MTT) assay was used to assess the proliferation effect s of resistin2132peptides. The apoptosis was investigated by the flow cy2 tometer, FCM. Results 　With the Resistin2132peptides t reatment of 50 、500 、5 000 and 10 000 ng/ ml, the inhibitory rate was 46 % to 92 %. The discrepancy between test and cont rol group s is statistically signifi2 cant ( P < 0. 01) . The apoptotic rate was 6. 7 %、6. 9 %、7. 5 % and 9. 8 % respectively. And the rate of cont rol group was 0. 6 % ( P < 0. 01) . Conclusion 　Resistin2132peptides can inhibit the proliferation of the cell proliferation and promote the apoptosis of the cells.
- Resistin /
- Breast neoplasm /
- Proliferation /
- Apoptosis