Abstract: Objective 　To study the effect s of Zoledronic acid on expression and secretion of VEGF in os2 teosarcoma LM8 cell line. Methods 　Af ter LM8 cells were t reated with Zoledronic acid, Semi2quantita2 tive PCR method was used to assess the expression of VEGF mRNA ; the expression and secretion of VEGF protein was detected by immunohistochemical staining and EL ISA respectively. Results 　The t ranscription of VEGF mRNA, expression and secretion of VEGF protein were found in LM8 cells. The t ranscription of VEGF mRNA, expression and secretion VEGF protein down2regulated by t reatment with 25μmol/ L, 50μmol/ L zoledronic acid for 24h and the effect s had a dose dependent relationship ( P <0. 05) . Conclusion 　Zoledronic acid can inhibit VEGF mRNA t ranscription, protein expression and secre2 tion in osteosarcoma LM8 cell line.