肠道脱落细胞的C-myc 癌基因检测在大肠癌诊断中的意义
Significance of C-myc Oncogene in the Colonic Exfoliative Cells in Colonic Carcinoma Dignosis
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摘要: 目的 探讨肠道脱落细胞中 C- myc癌基因检测在大肠癌诊断中的意义。方法 运用DNA点杂交法检测 39例大肠癌患者肠道脱落细胞及癌组织中 C- myc状态, 以正常人为健康对照。结果 58.9% ( 2 3/ 39)的癌症患者脱落细胞中存在 C- myc基因扩增, 64.1 % ( 2 5/ 39)的癌组织中存在 C- myc基因扩增。两者比较无明显差异 ( P>0 .0 5)。 1 0 % ( 2 / 2 0 )正常人脱落细胞中存在 C- myc扩增, 与病例组差异明显 ( P<0 .0 5)。大肠癌患者其脱落细胞 DNA C- myc扩增阳性率与发病部位和Dukes分期无明显相关 ( P>0 .0 5)。本法对大肠癌诊断的敏感性为 58.79%, 特异性为 90 %。结论 用 DNA点杂交法检测肠道脱落细胞中 C- myc癌基因是一种快速方便、经济可靠且非侵入性的诊断大肠癌方法, 可作为一种筛选试验.Abstract: Objective To investigate the significance of C myc oncogene in the colonic exfoliative cells in colonic carcinoma. Methods C myc oncogene was detected by DNA dot blot.39 cases with colonic carcinoma were diagnosed by pathology.20 cases controls without organic disease were excluded by colonic endoscopy. Results 0.7μg DNA was the best dose for detecting C myc oncogene by the hybridization.At this dose,C myc oncogene amplification could be seen in 58.9% (23/39) of the colonic exfoliative cells DNA and 64.1%...
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