Abstract: Objective 　To study on the relationship of virus infection, Tumor Necrosis ( TNF) and Soluble Interleukin 2 Receptor (SIL-2R) levels in patients with cervical cancer. Methods 　Virus infection were detected by Polymerase Chain Reaction ( PCR) and the levels of TNF, SIL-2R were measured by Enzyme Linked Immunosorbent Assay ( EL ISA) in 195 cases cervical cancer, and 75 cases benign diseases. Results 　The result showed that 152 in 195 cervical cancer cases were infected by virus (positive rates 77. 95 %) . The positive rates of Human Popillomavirus type 16, 18, 35 ( HPV16, 18, 35 ), Herpes Simplex Virus type 2 ( HSV Ⅱ) and Human Cytomegalovirus ( HCMV) were 31. % (62/ 195), 30. 76 % (60/ 195) and 3. 08 % (6/ 195) respectively. It s positive rates were significantly higher than that of the 20 %(15/75), 4. 0 % (3/ 75), 6. 67 % (5/ 75) and zero f rom 75 cases begin disease, ( P < 0. 01, respectively) . The result also showed the positive rates and levels of SIL22R and TNF were significantly higher in virus infection than that of the uninfection patient s with cervical cancer ( P < 0. 01, respectively) . Conclusion 　These result s suggest that the mixture infection of HPV16, 18, 35, HSV Ⅱand HCMV may play the cooperation role in inducing cervical cancer. The findings indicated virus infection may cause disorder and deficiency of immunity function, and variation of cytokines levels.