Abstract: Objective 　To investigate biological significance and relationship of ER、PR、p53 gene protein and DNA content in occurrence and development of gallbladder carcinoma. Methods 　The two steps of immunohistochemist ry and HPIAS-1000 DNA image inst rument were used to determine the expression of ER、PR、p53 and DNA content in 27 cases of gallbladder carcinoma. Results 　① In 27 cases of gallbladder carcinoma, the positive expression rates of the ER, PR, p53 were 77. 78 %、74. 07 % and 66. 67 %, respectively. ② The DNA ploid in high, middle and low differentiation gallbladder carcinoma were over doubleploid, ranging f rom over doubleploid to sub2quardriploid and sub2quardriploid, respectively. ③ In gallbladder carcinoma, with the increasing of the invasive depth and the lowering degree of differentiation, the positive expression rate of ER, PR and p53 were rising up, so was the DNA content s. Conclusion 　The positive expression ER、PR、p53 and DNA changeful ploidesis is related to the level of differentiate extent and infilt rated depth of gallbladder carcinoma. Excessive expression of ER、PR will possibly induce the mutein of p53 gene. They have closely inside relationship in the occurrence and development of gallbladder carcinoma .