大肠重复癌微卫星不稳定性研究

Microsatellite instability in multiple primary colorectal cancers

摘要: 目的　探讨微卫星不稳定性 (MSI)在大肠重复癌与单发大肠癌中的变化规律。方法　PCR SSLP法对 38例大肠重复癌患者的 5 1例癌灶及对照 35例单发大肠癌分别进行 5个碱基序列位点MSI检测。结果　大肠重复癌中复制错误 (RER)阳性率为 5 2 .9% (2 7/5 1), 对照组为 17.1% (6 /35 ), 两组差异有非常显著意义 (χ2 =11.2 5, P <0 .0 1)。大肠重复癌组中, RER阳性与低分化、近端大肠密切关联。RER阳性组与阴性组在年龄、性别、是否伴有转移、Dukes分期上未见异常。结论　MSI在大肠重复癌的发生上起着重要作用, MSI可作为预测大肠重复癌发生的有用标志, 对MSI大肠癌患者应警惕多重癌发生的可能性。

Abstract: Objective To study the changeable patterns of microsallite instability(MSI)in multiple primary colorectal carcinomas and solitary colorectal tumors. Methods MSI at five microsatellite loci were examined by PCR-SSLP method in 51 tumors from 38 patients with multiple primary colorectal cancers and 35 solitary colorectal tumors. Results The replication errors(RER)+ phenotype was observed in 27 of 51( 52.9% ) tumors from individuals with multiple primary colorectal cancers but only in 6 of 35( 17.1% ) tumor...