Abstract: Objective 　To investigate the lineage of H/ RS cell in classical Hodgkin lymphoma. Methods 　To analysis the expression of CD3 、CD20 、CD15 、CD30 、CD10 、MPO、bcl26 、κ mRNA andλ mRNA in 62 cases of H/RS cells in classical Hodgkin lymphoma by using immunohisto-chemical multiple staining technique and in situ hybridizations. Results 　In all 62 cases, the H/ RS cells were all positive for CD30, 41 cases positive for CD15, only 5 cases positive for CD20, all cases negative for CD3 、MPO、bcl-6 、CD10 、κmRNA andλmRNA. Conclusion 　H/ RS cells of classical Hodgkin lymphoma were originated f rom later stage germinal center developed B cells. H/ RS cells have great varies for B lineage markers . H/ RS cells were negative for immunoglobulinκandλdue to the neoplasm cells can't synthesize of immunoglobulin. H/ RS cells were not antigen driven, there have some unclearly mechanism in its hyperplasia.