细胞周期对肿瘤坏死因子诱导Hela 细胞凋亡的影响

Effect of Cell Cycle in Hela Cells to Apoptos is Induced by Tumor Necrosis Factor

摘要: 目的　研究细胞周期对肿瘤坏死因子 ( TNF)诱导 Hela细胞凋亡的影响。方法　通过胸腺嘧啶核苷酸 ( Td R)阻断法阻滞 Hela细胞的细胞周期, 以 MTT法、流式细胞术和荧光染色分析 Td R阻滞细胞和周期化的 Hela细胞对 TNF诱导凋亡的敏感性。结果　Td R阻滞细胞周期较周期化的 Hela细胞对 TNF诱导的凋亡的敏感性降低。结论　揭示 TNF诱导 Hela细胞凋亡与细胞周期有关.

Abstract: Objective To study effect of cell cycle in Hela cells to apoptosis induced by tumor necrosis factor. Methods Hela cells was arrested by cell cycle blocking drug TdR.Hela cells in arresting and cycling were treated with tumor necrosis factor.Cell apoptosis was evaluated by MTT assay,flow cytometry and fluorescent staining. Results Hela cells by cell cycle arrest became lower sensitve to TNF induced apoptosis. Conclusion It suggested that Hela cells to apoptosis induced by tumor necrosis factor may be related ...