PTEN在胃癌细胞中的表达及其CpG岛甲基化状态的研究

Studying the Expression of PTEN and Its CpG Island Methylation Status in Gastric Carcinoma Cells

摘要: 目的检测抑癌基因PTEN在四种胃癌细胞中mRNA和蛋白表达水平及其5'启动子区CpG岛甲基化状态。方法采用甲基化特异性聚合酶链反应(Methylation-specific PCR,MSP)检测四种不同分化程度的胃癌细胞(HGC-27,MGC-803,BGC-823,SGC-7901)中PTEN基因启动子区域甲基化状态,RT-PCR和Westernblot法分别检测其mRNA和蛋白表达水平。结果除SGC-7901外,其他三种细胞可检测到PTEN基因启动子的甲基化。PTENmRNA和蛋白表达水平依次为:SGC-7901最高(P<0.01),BGC-823、MGC-803次之(两者间无显著性差异,P>0.05),HGC-27表达水平最低(P<0.01),其表达与细胞分化程度呈正相关趋势。结论PTEN基因启动子区异常甲基化可能导致该基因转录表达失活,使其蛋白表达减少甚至缺失,这可能是导致胃癌发生、发展的原因之一。

Abstract: Objective To detect hypermethylation status of the 5' CpG island locating in the promoter region of PTEN gene and the expression level of their mRNA and protein in 4 gastric carcinoma cells. Methods Using methylation-specific PCR(MSP) technique to detect methylation status of PTEN gene in 4 different differentiation gastric cancer cells(HGC-27,MGC-803,BGC-823,SGC-7901),RT-PCR and Western blot technique to detect their mRNA and protein expression level. Results Except SGC-7901,other three cells were detected...