Abstract: Objective 　To investigate the inhibition of the expression of RON gene and cell growth in lung cancer cells A549 by the antisense nucleic acid t reatment, and explore the possibility of the antisense nucleic acid of gene RON as a target for lung cancer gene therapy. Methods 　Transmembrance domain of RON gene (RONm) was obtained and inversely inserted in eukaryotic expression vector, obtain the replication deficient pcDNA by transfection the pack-ing cells of BJ5183, then infect the A549 cells by the anti-sense nucleic acid. Cell of lung cancer was transfected with this vector. The expression of RON gene in model was dectec-ted by EL ISA and the alteration of cell biology activity was determined by MTT. Results The amount of RON depression and the cell biology activity of the transfected A549 were depressed when compared with the control cell lines. Conclusion 　The anti-sense nucleic acid of RONm should significantly inhibit the expression of RON gene and arrest the growth of A549.