组织芯片简易制作方法及免疫组化有效性分析
A Simple Making Method of Tissue Microarray and Validation Study on Immunohistochemistry
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摘要: 目的 探索一种组织芯片的简易制作方法,并对它的有效性进行分析。方法 在实验中探索出组织芯片的制作方法,收集乳腺癌病例124例,制作成直径1.6cm的组织芯片,进行雌激素受体（ER）、孕激素受体（PR）及c-erBb2受体免疫组化染色,同时与普通切片的免疫组化染色结果对比,并进行统计学分析。结果 组织芯片与普通切片的ER、PR及c-erBb2染色积分（0～7分）显著相关,ER、PR及c-erBb2的表达（阳性/阴性）一致率分别达94.35%、93.55%和91.94%。比较组织芯片与普通切片的ER、PR及c-erBb2的表达,两者之间无统计学差异（P〉0.05）,即组织芯片的结果与普通切片的结果是一致的。结论 自制1.6mm直径的组织芯片在进行免疫组化染色时能够代表普通切片,自制组织芯片的方法可靠有效。Abstract: Objective To search a simple making mothd of tissue microarray and evaluate it s validation. Methods A new, simple and easy method was designed to improve the technology of TMA during the experiment . Tissue microarrays with 1. 6 mm in diameter tissue core were const ructed from 124 cases of breast cancer. The tissue microarrays were stained for oest rogen receptor ( ER), progesterone receptor (PR) and c-erBb2 using immunohistochemistry. At the same time the stains on the tissue microarrays were compared with that from the full tissue sections. Results A highly significant association was observed between the staining scores (0 ～ 7) obtained f rom the full tissue sections and f rom the tissue microarrays. Concordance for the receptor status (positive / negative) of the whole section and the tissue core were 94. 35 % for ER, 93. 55 % for PR. and 91. 94 % for c-erBb2. The discordance between full section and tissue microarrays was studied using Two 2 Related 2 Samples tests and the result had no statistical significance ( P > 0. 05) . Conclusion Tissue microarays with 1. 6 mm in diameter tissue core made by simple method can represent full tissue section on immunohistochemistry study. We believe our method of tissue microarray is a reliable and valid
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