Abstract:
Objective To investigate the expression of ezrin gene in some carcinoma cells,to detect the transcriptional activity of its 5′-flanking region,and to elucidate the transcriptional regulatory mechanism of the ezrin gene in carcinoma cells. Methods Expression of ezrin gene in esophageal carcinoma EC109,EC171,EC8712 and SHEEC,gastric carcinoma N87 and BGC823,lung carcinoma A549 and 95D,hepatocellular carcinoma HepG2,leukemia K562,and cervical carcinoma HeLa cells was detected using reverse transcriptase polymerase chain reaction (RT2PCR) and western blot analysis. Plasmid p GLB2hE( -1444/ + 134), containing - 1444/ + 134 of ez rin gene fused to the luciferase gene, was generated by PCR method. Transcriptional activity of the - 1444/ + 134 region in some carcinoma cells was examined using dual2luciferase reporter assay system. Results All the tested carcinoma cells high2expressed ez rin mR2 NA and Ezrin protein. - 1444/ + 134 region of ez rin gene had st rong t ranscriptional activity compared with SV40 promoter ( P < 0. 05) in EC109, Hela, A549 and BGC823 cells. Conclusion The t ranscrip2 tional activity of the ez rin gene
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