雌激素诱发大鼠催乳素瘤的机制

徐　春; 王　晖; 刘晓军

doi:10.3971/j.issn.1000-8578.1202

雌激素诱发大鼠催乳素瘤的机制

1. 100039 北京,武警总医院内分泌科;2. 武警烟台疗养院;3. 武警总医院药剂科

详细信息

中图分类号: R736. 4
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出版历程
- 收稿日期: 2005-12-07
- 修回日期: 2006-02-19
- 刊出日期: 2007-01-04

The Mechanism of Prolactinomas Induced by Estrogen in Rats

XU Chun¹,
WANG Hui²,

1. Department of Endocrinology, General Hospi tal of Armed Police Forces, Bei jing 100039, China;2. Yantai Sanatorium of Armed Police Forces

摘要

摘要: 目的通过分析雌激素对大鼠垂体Pit-1基因表达的作用，探讨雌激素诱发大鼠催乳素瘤的发病机制。方法成年雌性Wistar大鼠，去势手术后分2组：（1）对照组（C组，n=16）皮下植入空白硅胶管；（2）雌激素组（E组，n=16）：皮下植入含有乙稀雌酚的硅胶管。分别于用药第2、4、6、8周处死动物。垂体称重。用放免法测定大鼠血清PRL水平。用反转录-PCR方法检测Pit-1 mRNA在各组垂体组织中的表达量。结果在用药第2、4、6、8周，大鼠血清PRL水平、垂体重量、垂体组织Pit-1 mRNA水平均逐渐增高，垂体组织Pit-1 mRNA水平与大鼠血清PRL水平和垂体重量均呈明显正相关（相关系数分别为0．946和0．913，P=0．01）。结论雌激素刺激垂体组织表达Pit-1基因，Pit-1在雌激素诱发的大鼠PRL瘤的发生中起重要作用。
- 发病机制 /
- 大鼠 /
- 催乳素瘤 /
- 雌激素 /
- Pit-1
Abstract: Objective 　To study the mechanism of prolactinomas induced by estrogen in rats. Methods 　Adult Wistar rats were divided into 2 groups at random. Each rat in cont rol group ( n = 16) were subscutaneously implanted with a blank implant . Rats in estrogen group ( n = 16) were implanted with est rogen-containing implants. The animals were executed af ter 2, 4, 6, 8 weeks. Serum prolactin ( PRL ) levels were measured by RIA method. Pit-1 mRNA level in pituitary tissue were measured by RT-PCR method. Results 　Pit-1 mRNA levels were different among 2, 4, 6, 8 weeks and increased along with 2, 4, 6, 8 weeks. There was a positive relation between Pit-1 mRNA levels and serum PRL levels (γ= 0. 964, P = 0. 01) . There was also a positive relation between Pit-1 mRNA levels and pituitary weight s (γ= 0. 913, P = 0.01) . Conclusion 　Estrogen has a stimulating effect on the expression of Pit-1 gene, which plays an important role on est rogen induced prolactinoma.
- Mechanism /
- Rats /
- Estrogen /
- Prolactinomas /
- Pit-1