PKC抑制剂对人肺腺癌细胞的放射增敏作用

Radiosensitization Effect of PKC Inhibitor on Human Lung Adenocarcinoma Cell Line

摘要: 目的　探讨PKC抑制剂对肺癌放射敏感性的影响；方法　采用MTT法检测了人肺腺癌细胞株A549在PKC抑制剂白屈莱红碱(CH)处理前后，2Gy照射的细胞存活分数(SF2)，并采用流式细胞术，对其凋亡率以及p53、Bcl -2蛋白表达进行了检测；结果　CH处理后A549凋亡率增高，SF2下降。p53在X线照射后表达增强，而CH处理组p53表达呈相对抑制状态。Bcl-2在处理前后变化温和。结论　PKC抑制剂对A 549细胞株的放射敏感性有增强作用，这种作用可能与凋亡率升高有关，而p53可能与 PKC抑制剂诱导的凋亡无关。

Abstract: Objective　Chelerythrine(CH),a specific inhibitor of protein kinase C-α、β，on the cell radiosensitivity (surviving f raction at 2Gy,SF2)，expression of the p53、Bcl-2 protein,and apoptosis of human lung adenocarcinoma cell line A549 was studied.Treat A549 cells with different concentrations of CH enhanced X-ray induced cell apoptosis, and decrease the SF2，the expression of p53 but not Bcl-2 protein was obv iously effected by CH. Conclusion 　The PKC inhibitor sensitize the A549 cells to ionizing radiation,it may not depend on the quantity of th e p53 protein expression.