人源抗肝癌单链抗体库的构建及初步鉴定
Construction and Preliminary Identification of Single-chain Fv Antibody against Human Hepatocellular Carcinoma
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摘要: 目的构建人源抗肝癌单链抗体基因噬菌体表面呈现文库。方法利用噬菌体表面呈现技术,构建基因文库,经过panning筛选富集后,用ELISA方法检验抗原结合活性。结果从30个噬菌体克隆中筛选到8个具有肝癌细胞株SMMC7721结合活性的阳性克隆。结论从外周血淋巴细胞中获取可变区基因,利用噬菌体抗体库技术制备人源抗肝癌单链抗体的策略是可行的。Abstract: Objective To construct the phage display library of anti-human hepatocellular carcinoma ScFv. Methods The ScFv gene library was constructed by phage display technology. Binding activity of recombinant scFv was detected by ELISA after panning. Results Eight antigen binding clones were identified from 30 individual phagemid clones. Conclusion In the construction of engineering antibodies against human hepatocellular carcinoma, the strategy of phage display technique and of RT-PCR from peripheral blood lymphocy...
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