CCNG2 Expression and Its Effect on Proliferation and Apoptosis of Thyroid Papillary Cancer K1 Cells

Objective To examine the effect of CCNG2 on the proliferation and apoptosis of thyroid papillary carcinoma (PTC) K1 cells.

Methods In the case of CCNG2's overexpression in PTC K1 cells, we built cells line by means of transient transfection technology, observed the proliferation and apoptosis of cells by MTT assay and flow cytometry (FCM), and detected the protein expression of CCNG2, P53 and MDM2 by Western blot and immunohistochemistry.

Results MTT assay and flow cytometry (FCM) indicated that CCNG2 overexpression significantly inhibited the proliferation and induced the apoptosis of K1 cells (P < 0.05); Western blot analysis showed that tumor suppressor gene P53 protein expression was significantly raised in K1 cells with CCNG2 gene overexpression(P < 0.05); the immunohistochemistry showed the same result with Western blot analysis.

Conclusion CCNG2 can inhibit the proliferation and promote the apoptosis of PTC K1 cells partly by increasing the protein expression level of P53.