Anti-mouse Hepatoma Effect of Tumor-infiltrating Lymphocyte Stimulated by DCs Pulsed with H22 Full-cell Antigen

Objective To study the anti-mouse hepatoma effect of tumor-infiltrating lymphocytes (TILs) stimulated by dendritic cells (DCs) pulsed with the tumor full-cell antigen in vitro. Methods DCs were isolated from BALB/c mouse bone marrow and stimulated by granulocyte macrophage colony stimulating factor (GM-CSF),interleukin-4(IL-4) and H22 full-cell antigen.The maturation surface markers CD80，CD86，CD40，MHCⅡ and CD11c were detected by flow cytometry before and after pulsing with whole H22 cells lysates.Then the cytotoxic potency of the TILs was assessed both stimulated and unstimulated,and taking the spleen lymphocytes as control groups.ResultThe expression of CD80，CD86，CD40，MHCⅡon the DCs pulsed with H22 full-cell lysate is a higher than the DCs after 5d of culture.TILs stimulated by the DCs on mouse hepatoma H22 cells had very special efficient anti-tumor effect on the mouse liver cancer H22 in vitro.And the anti-tumor effect of stimulated TIL is higher than the TILs and spleen lymphocytes. Conclusion The results suggest that H22 tumor full-cell antigens specific induced tumor-infiltrating lymphocytes have more efficient at killing the H22 cells.