脑胶质瘤IL-6/IL -6R 自分泌或旁分泌环路的存在及意义

The Autocrineor ParacrineInterleukin-6/Interleukin-6 Receptor Loop In Gliomas

摘要: 目的　研究脑胶质瘤中IL 6 /IL 6R自分泌或旁分泌环路的存在和意义。方法　采用逆转录聚合酶链反应 (RT PCR)方法检测白细胞介素 6 (IL 6 )和IL 6受体 (IL 6R)基因在脑胶质瘤细胞株、脑胶质瘤组织标本和正常人脑胶质细胞中的表达。结果　 2株脑胶质瘤细胞株均有IL 6和IL 6R的表达, 5 2例脑胶质瘤组织标本中有 38例 (73.1% )表达IL 6、4 4例 (84 .6 % )表达IL 6R、33例 (6 3.5 % )同时表达IL 6和IL 6R。人脑正常胶质细胞仅有IL 6基因弱表达, 而无IL 6R表达。结论　脑胶质瘤可能存在IL 6 /IL 6R自分泌或旁分泌环路, 此环路与肿瘤的恶性增殖有关。

Abstract: Objective To evaluate the significance of the autocrine of paracrine interleukin-6/interleukin-6 receptor loop in gliomas. Methods We detected IL-6 and IL-6R gene in adult cerebral tissue, cerebellar tissue, 2glioma cell lines and 52 human glioma tissue specimens with different malignant degree. A complementary DNA copy of total RNA was synthesized and amplified with specific primers using the reverse transcription polymerase chain reaction(RT-PCR)method. Results The experiments revealed that both C6 and ...