三氧化二砷对非M_3的AML及ALL细胞Fas和FasL蛋白影响的研究
Effect of Arsenic Trioxide on Fas/FasL in Human Non-M3 AML and ALL Cell Lines
-
摘要: 目的 了解As2 O3 对非M3 的急性髓性白血病 (AML)及急性淋巴细胞性白血病 (ALL)细胞Fas、FasL蛋白的影响。方法 将非M3 的AML细胞和ALL细胞体外与不同浓度As2 O3 共同培养, 通过细胞计数、细胞活力的判定并利用流式细胞仪检测DNA、Fas和FasL蛋白的变化, 来观察As2 O3 对上述细胞的细胞活力、增殖周期及Fas、FasL蛋白的影响。结果 随着As2 O3 浓度由 0 μmol/L增加至2 μmol/L, 细胞活力由 (91.1± 2 .3) %降至 (6 4 .32± 3.5 ) % ;细胞生长抑制率由 (10 .6± 1.6 ) %增至(2 9.36± 2 .6 ) % ;细胞凋亡率由 (0 .0 7± 2 .5 ) %增至 (5 .0 4± 2 .2 ) % ;随着As2 O3 浓度增加, Fas、FasL的阳性率也随之增加。结论 As2 O3 以浓度依赖的方式抑制非M3 的AML及ALL细胞的生长、活力及细胞周期, 使G0 G1期细胞增多, As2 O3 在诱导非M3 的AML及ALL细胞发生凋亡的同时, 可以上调Fas、FasL的表达, 提示Fas/FasL系...Abstract: Objective To investigate the effect of As 2O 3 on Fas/FasL in human non M 3 AML and ALL cells. Methods We cultured non M 3 AML and ALL cells with different dose As 2O 3 together in vitro.The effects on cells living and growth were evaluated according to cell counting and determining of cell living.Meanwhile flow cytometry was used to detect the change of DNA,Fas and FasL. Results As the dose of As 2O 3 increased from 0μmol/L to 2μmol/L,the rates of inhibition of non M 3 AML and ALL cell growth ...
下载:
