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转录因子Snail对食管癌Eca-109细胞侵袭和迁移的影响

李文雯, 张琳丽, 胡国清

李文雯, 张琳丽, 胡国清. 转录因子Snail对食管癌Eca-109细胞侵袭和迁移的影响[J]. 肿瘤防治研究, 2015, 42(04): 319-323. DOI: 10.3971/j.issn.1000-8578.2015.04.001
引用本文: 李文雯, 张琳丽, 胡国清. 转录因子Snail对食管癌Eca-109细胞侵袭和迁移的影响[J]. 肿瘤防治研究, 2015, 42(04): 319-323. DOI: 10.3971/j.issn.1000-8578.2015.04.001
LI Wenwen, ZHANG Linli, HU Guoqing. Effect of Transcription Factor Snail on Invasion and Migration of Esophageal Carcinoma Cell Line Eca-109[J]. Cancer Research on Prevention and Treatment, 2015, 42(04): 319-323. DOI: 10.3971/j.issn.1000-8578.2015.04.001
Citation: LI Wenwen, ZHANG Linli, HU Guoqing. Effect of Transcription Factor Snail on Invasion and Migration of Esophageal Carcinoma Cell Line Eca-109[J]. Cancer Research on Prevention and Treatment, 2015, 42(04): 319-323. DOI: 10.3971/j.issn.1000-8578.2015.04.001

转录因子Snail对食管癌Eca-109细胞侵袭和迁移的影响

基金项目: 国家自然科学基金(81272491)
详细信息
    作者简介:

    李文雯(1986-),女,博士在读,主要从事肿瘤侵袭转移、放射生物学相关研究

    通讯作者:

    胡国清,E-mail: gqhu@tjh.tjmu.edu.cn

  • 中图分类号: R735.1

Effect of Transcription Factor Snail on Invasion and Migration of Esophageal Carcinoma Cell Line Eca-109

  • 摘要: 目的 探讨Snail在诱导食管癌细胞侵袭和迁移中的作用及机制。方法 采用慢病毒转染构建食管癌细胞株Eca-109-Snail和Eca-109-vc,Real-time PCR及Western blot实验分别检测其mRNA及蛋白表达水平,Transwell及细胞划痕实验检测细胞侵袭和迁移能力。 结果 慢病毒转染细胞株构建成功。经Real-time PCR及Western blot实验鉴定慢病毒转染后的Eca-109-Snail细胞Snail表达量升高,细胞表现出成纤维细胞样外形,细胞之间彼此分离;Eca-109-Snail细胞E-cadherin表达下降,Vimentin和MMP-2表达上升,其侵袭和迁移能力较空载体对照组增强,差异均具有统计学意义(P<0.01)。 结论 Snail通过诱导食管癌Eca-109细胞发生上皮间质转化,进而提高其侵袭和迁移能力。

     

    Abstract: Objective To investigate the effect of Snail on the invasion and migration of esophageal carcinoma cell line and the potential mechanism in vitro. Methods Esophageal carcinoma cell line Eca-109-Snail and Eca-109-vc were constructed by recombinant lentiviral transduction. The mRNA and protein expression of Snail were detected by Real-time PCR and Western blot. Transwell assay and wound healing assay were performed to investigate cells invasion and migration capability. Results Two stable cell lines were established. Increased expression of Snail mRNA and protein in Eca-109-Snail were detected by Real-time PCR and Western blot. The phenotypic changes of spindle-cell morphology and increased intercellular separation were noted in Eca-109-Snail cells. Down-regulation of E-cadherin and up-regulation of Vimentin and MMP-2 were observed in Eca-109-Snail cells. The invasion and migration capability of Eca-109-Snail cells were higher than those of Eca-109-vc cells, with statistically significant difference (P<0.01). Conclusion Snail promotes the invasion and migration via inducing epithelial-mesenchymal transition in Eca-109 cells in vitro.

     

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出版历程
  • 收稿日期:  2014-09-09
  • 修回日期:  2014-12-15
  • 刊出日期:  2015-04-24

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