高级搜索

RNA干扰沉默α1,3岩藻糖转移酶-Ⅶ基因对人肝癌细胞增殖的影响

佘尚扬, 蓝秀万, 何 敏, 王秋雁

佘尚扬, 蓝秀万, 何 敏, 王秋雁. RNA干扰沉默α1,3岩藻糖转移酶-Ⅶ基因对人肝癌细胞增殖的影响[J]. 肿瘤防治研究, 2010, 37(05): 507-510. DOI: 10.3971/j.issn.1000-8578.2010.05.005
引用本文: 佘尚扬, 蓝秀万, 何 敏, 王秋雁. RNA干扰沉默α1,3岩藻糖转移酶-Ⅶ基因对人肝癌细胞增殖的影响[J]. 肿瘤防治研究, 2010, 37(05): 507-510. DOI: 10.3971/j.issn.1000-8578.2010.05.005
SHE Shang-yang, LAN Xiu-wan, HE Min, WANG Qiu-yan. Effect of α1,3-fucosyltransferase-Ⅶ Gene Silence by RNA Interference on Cell Proliferation of Human Hepatocarcinoma Cells[J]. Cancer Research on Prevention and Treatment, 2010, 37(05): 507-510. DOI: 10.3971/j.issn.1000-8578.2010.05.005
Citation: SHE Shang-yang, LAN Xiu-wan, HE Min, WANG Qiu-yan. Effect of α1,3-fucosyltransferase-Ⅶ Gene Silence by RNA Interference on Cell Proliferation of Human Hepatocarcinoma Cells[J]. Cancer Research on Prevention and Treatment, 2010, 37(05): 507-510. DOI: 10.3971/j.issn.1000-8578.2010.05.005

RNA干扰沉默α1,3岩藻糖转移酶-Ⅶ基因对人肝癌细胞增殖的影响

详细信息
    通讯作者:

    王秋雁

  • 中图分类号: R735.7

Effect of α1,3-fucosyltransferase-Ⅶ Gene Silence by RNA Interference on Cell Proliferation of Human Hepatocarcinoma Cells

More Information
    Corresponding author:

    WANG Qiu-yan

  • 摘要: 目的:研究RNA干扰沉默α1,3 岩藻糖转移酶-Ⅶ (α1,3FucT-Ⅶ) 基因对人肝癌细胞增殖的影响。方法:设计合成α1,3FucT-Ⅶ特异性RNA干扰质粒,并用脂质体转染导入人肝癌H7721细胞。RT-PCR检测细胞中α1,3FucT-Ⅶ mRNA的表达。流式细胞术测定细胞表面唾液酸化的Lewis X抗原(SLex)的表达及细胞周期。细胞生长曲线通过MTT测定。p27Kip1的表达检测采用Western blot。结果:成功构建了α1,3FucT-Ⅶ特异的shRNA真核表达质粒载体。RNA干扰下调α1,3FucT-Ⅶ表达能抑制H7721细胞生长,表现为细胞生长速度明显较对照组细胞慢,S期细胞百分数明显降低,p27Kip1的表达增加。结论:α1,3FucT-Ⅶ可能通过调节p27Kip1的降解影响H7721细胞增殖。

     

    Abstract: Objective:To study the effect of α1,3 fucosyltransferase-Ⅶ (FucT-Ⅶ) gene silence on the hepatocarcinoma cell proliferation. Methods:α1,3FucT-Ⅶ specific RNA plasmid vector was constructed and then transfected into human H7721 hepatocarcinoma cell line with lipofectamine 2000. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the level of α1,3FucT-Ⅶ mRNA. Cell surface SLex and cell cycles were analyzed by flow-cytometry. Cell growth was detected by MTT method. The expression of p27 Kip1 was examined using western blot. Results:The specific shRNA targeted to α1,3FucT-Ⅶ gene could down regulate the expression of α1,3FucT-Ⅶ and inhibit the proliferation of H7721 cells. It was evidenced by decreasing the rate of cell growth and blocking the cells going from G1 to S stage, as well as increasing the expression of p27Kip1. Conclusion:α1,3FucT-Ⅶ may affect cell proliferation of H7721 cells by regulating the degradation of p27Kip1.

     

计量
  • 文章访问数:  2163
  • HTML全文浏览量:  14
  • PDF下载量:  576
  • 被引次数: 0
出版历程
  • 收稿日期:  2008-12-28
  • 修回日期:  2009-08-16
  • 刊出日期:  2010-06-24

目录

    /

    返回文章
    返回
    x 关闭 永久关闭