Abstract: Objective：To design and synthesis siRNAs targeting Bone Morphogenetic Protein2(BMP-2) gene and to observe its influence on the proliferation and apoptosis of SMMC7721 cells. Methods：Three specific siRNAs of BMP-2 were designed and synthesized,and were transiently transfected into SMMC7721 via cathodolyte liposome transfection method. Reverse transcriptasePCR and Western blotting were used to measure BMP-2 expression at mRNA and protein level,respectively. The cell proliferation was assessed by MTTassay and the changes in cell cycle and apoptosis were evaluated by flow cytometry. Results：All the 3 specific BMP-2-siRNA(A,B,C)could effectively inhibit the expression of BMP-2 gene, BMP-2-siRNA-B had the highest inhibitive effect.The proliferation of SMMC7721 cells was markedly inhibited and the apoptosis of SMMC7721 cells was increased transfected by BMP-2-siRNAs( P <0.05). Conclusion：The siRNA of BMP-2 could effectively inhibit the expression of BMP-2 and induce the growth inhibition and apoptosis increase in SMMC7721 cells.