siRNA干扰ERK1/2表达对食管癌细胞 ezrin 基因的转录调控作用

Effect of ERK1/2 siRNA on Transcriptional Regulation ofezrinin Esophageal Carcinoma Cells

摘要: 目的：检测siRNA干扰细胞外信号调节激酶ERK1/2基因表达对 ezrin 基因的转录调控作用，探讨食管癌细胞 ezrin 基因的表达调控机制。方法：采用定量RT-PCR技术，检测转染ERK1/2 siRNA对食管癌EC109细胞ERK1/2和 ezrin 基因mRNA表达水平的影响；采用双荧光素酶报告基因分析系统，检测siRNA干扰ERK1/2表达对EC109 细胞 ezrin 基因启动子活性的影响。结果：在食管癌EC109细胞中，转染ERK1/2 siRNA，降低ERK1/2和 ezrin 基因的mRNA表达水平以及 ezrin 基因启动子活性。结论：ERK1/2对食管癌细胞 ezrin 基因的转录具有调控作用。

Abstract: Objective：To identify the effect of ERK1/2 siRNA on the transcriptional regulation ofezringene and elucidate the transcriptional regulatory mechanism ofezrinin human esophageal carcinoma cells. Methods：Esophageal carcinoma EC109 cells were transfected with ERK1/2 siRNA or control siRNA, and the effect of siRNA on ERK1/2 andezringenes mRNA expression was determined by real time RT-PCR. Furthermore, the role of ERK1/2 siRNA interference inezrinpromoter activity was analyzed by dual-luciferase reporter assay system. Results：Transfection of ERK1/2 siRNA to EC109 cells decreased the mRNA expression of ERK1/2 andezringenes and theezrinpromoter activity. Conclusion：ERK1/2 regulateezringene transcription in human esophageal carcinoma cells.