多聚乙烯亚胺介导小鼠IL-12 基因转染人骨肉瘤细胞的细胞

Polythylenimine AS A Nonviral Vector for Murine Interleukin-12 Gene Transfer to the Mouse Osteosarcoma Cells

摘要: 目的确定多聚乙烯亚胺(polyethylenimine，PEI)最佳的转染条件。以PEI为载体，将mIL-12基因导入MG63细胞。方法以MTT法检测PEI的细胞毒性。以PEI为载体，在不同的条件下．将荧光素酶基因和mIL-12基因导入MG63细胞，分别检测其转染效率。结果 PEI的细胞毒性较低。当N／P比值为8时,PEI的转染效率最高。PEI可以成功的将mIL-12基因导入MG63细胞。结论 PEI是一种低毒性、高效率的DNA载体，可以广泛应用于体内外基因治疗的试验。

Abstract: Objective 　To investigate the best condition of polyethylenimine ( PEI) for murine interleukin-12 gene transfer to MG63 cells. Methods 　The cytotoxicity of PEI was evaluated by MTT. In different conditions, using PEI as the vector, MG63 cells were t ransfected with luciferase gene and murine interleukin-12 (mIL-12) gene. Expression efficiency of the transduced gene was monitored. Results 　The cytotoxicity of PEI was low. When N/ P ratio equalled to 8, the t ransfection efficiency was highest, and mIL-12 gene could also be successfully t ransferred to MG63 cells with PEI as the vector. Conclusion 　PEI is low cytotoxicity, and high efficiency vector. It can be widely used in the area of gene therapy.