Synergistic Inhibitory Effect of survivin siRNA in Combination with 5-Fu on Inhibiting Proliferation of MCF-7 Cells
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摘要: 目的研究以survivin为靶标的小干扰RNA(siRNA)与化疗药5-Fu联合应用抑制MCF-7细胞增殖的作用。方法以脂质体为载体,将survivin siRNA转染至MCF-7细胞中,用四氮唑盐(MTT)法染色并计算siRNA联用5-Fu对MCF-7细胞的抑制率,用SAS统计软件及金正均Q值法进行统计分析。结果单用5-Fu,IC50为4.42μg/ml;加入5nmol/LsiRNA后,IC50降为1.18μg/ml;siRNA与5-Fu联用的抑制作用较单用5-Fu强(F=26.74,P<0.01);Q值分析表明survivin siRNA与中低浓度的5-Fu联用,有较好的协同作用(Q≥1.15)。结论survivinsiRNA与5-Fu联用,可显著增强对MCF-7细胞增殖的抑制,提高肿瘤细胞对化疗药物的敏感性。Abstract: Objective To investigate the effect of the small interfering RNA(siRNA) targeted to survivin in combination with 5-Fu on inhibition the of MCF-7 cells proliferation. Methods A siRNA targeted to survivin was synthesized.siRNA was transfected into MCF-7 by lipofectin.Cell growth activity was evaluated by MTT assay.SAS software and Jin Zhenjun Method were used to evaluate the combination effects of siRNA and 5-Fu. Results Combination treatment with 5 nmol/L siRNA reduced the IC50 of 5-Fu from 4.42 μg/ml to 1.18 18μg/ ml ; the inhibitory of combination t reatment on MCF27 cells was higher than that of 5-Fu alone ( F = 26. 74, P < 0. 01. And synergism(Q ≥1. 15) was observed at the lower concent ration of 52Fu with combination of siRNA. Conclusion siRNA may enhance the effectiveness of 5-Fu on inhibiting the proliferation of MCF-7 cells.
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Key words:
- RNA interfer /
- Chemotherapy /
- Combination therapy /
- MCF-7
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