Abstract: Objective 　To study the expression of Neuropilin-1 in human pancreatic carcinoma specimens and cell lines. Methods 　We investigated the expression of Neuropilin-1 in human pancreatic carcinoma specimens by immunohistochemical staining and examined the expression of Neuropilin-1 mRNA in human pancreatic carcinoma specimens and cell lines using RT-PCR. Results 　Immunohistochemical analysis revealed that NRP-1 was expressed in 14 of 14 human pancreatic adenocarcinoma specimens but was absent in nonmalignant pancreatic tissue. It can be detected the mRNA of NRP21 in 9 of 14 human pancreatic carcinoma specimens and Panc-1 、Aspc-1 、Bxpc-3 cell lines while can not be detected in nonmalignant pancreatic specimens. Conclusion 　Neuropilin-1 was expressed in most of human pancreatic carcinoma specimens and cell lines but not expressed in nonmalignant pancreatic tissue. It suggested the the expression of Neuropilin-1 may be relevant to the risk and development of pancreatic carcinoma.